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and then with diluent (1×) Measure linearity by dilating the sample to within the detection range
C5a is an extremely potent proinflammatory mediator
low-speed centrifuge
cellular supernatant Serum/plasma 100 ul cells that add 20 ul supernatant fluid sample activation fluid or（1 N HCl） 40uL serum/plasma added to 20uL sample activation solution or（1 N HCl） Blending and incubation for 10 min at room temperature Blending and incubation for 10 min at room temperature Add 20 ul activated terminated liquid or（1
FITC Mouse Anti-Human CD154 Antibody (S-R579) Size:100T and then with diluent (1×)Product Specification Host Mouse Antigen CD154 Synonyms CD40 ligand; CD40 L; T cell antigen Gp39; TNF related activation protein (TRAP); Tumor necrosis factor ligand superfamily member 5; CD40L; TNFSF5; TRAP; CD40LG Location Cell membrane Accession P29965 Clone Number S R579 Antibody Type Mouse mAb Isotype IgG1,k Application FCM Reactivity Hu Purification Protein G Conjugation FITC Physical Appearance Liquid Storage Buffer PBS, 25% Glycerol, 1% BSA,
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