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Conjugation Unconjugated Tag GST Tag Physical Appearance Liquid Storage Buffer 50 mM Tris-HCl (pH 8
and reverse transcription of cDNA above 6 kb is recommended for 60 min
so it is recommended to open the advanced structure of the RNA 5' end by thermal denaturation (heating at 65°C for 5 min
The recombinant HA tag fusion proteins can be purified directly from pre-treated cell lysate using Rabbit Anti-HA Agarose Beads
Mouse INOS ELISA Kit Size:96T Conjugation Unconjugated Tag GST TagProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and