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Principle: The NEK7 assay is performed using the ADP-GloTM Kinase Assay kit which quantifies the amount of ADP produced by the NEK7 reaction
Constituent Dosage PB 855μL CU 40μL AC 100μL 594 primary colour 5μL Reaction liquid volume 1000μL
gently mix (concentration is 20 ng/mL)
Avoid any cell stimulation during the procedure
PerCP-Cy5.5 Mouse Anti-Human CD33 Antibody (S-R472) Multiplex Kit Principle: The NEK7 assay isProduct Specification Host Mouse Antigen CD33 Synonyms Myeloid cell surface antigen CD33; Sialic acid binding Ig like lectin 3 (Siglec 3); gp67; SIGLEC3 Location Cell membrane Accession P20138 Clone Number S R472 Antibody Type Mouse mAb Isotype IgG2a,k Isotype Control S0B80056 Application FCM Reactivity Hu Positive Sample Human PBMC Purification Protein A Concentration 0. 2 mg ml Conjugation PerCP Cy5. 5 Physical Appearance Liquid Storage Buffer PBS,