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and HRP conjugate are sequentially added to microwells pre-coated with a voltage-dependent anion-selective channel 1 (VDAC1) capture antibody
Interleukin-1 beta (IL-1β) is a proinflammatory cytokine that is mainly produced by monocytes and activated macrophages as a proprotein
airway smooth muscle cells and endothelial cells
and it can be released by MMP mediated shedding
Bovine CD63 ELISA Kit IVD Antigen Recombinant Protein and HRP conjugate are sequentiallyProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample handling and requirements: Plasma: Collect specimens using EDTA or heparin as an anticoagulant. Centrifuge at 1000g for 15 minutes at 2 8C within 30 minutes of collection. Remove the supernatant for analysis or store at 20C