1-N-Me-Pseudo UTP, 100mM Solution Application_mRNA " Nucleic acids research 30

" Nucleic acids research 30

THE JOURNAL OF BIOLOGICAL CHEMISTRY Vol

5 at 25℃)

DNase I (RNase free) is an endonuclease that digests single- and double-stranded DNA

1-N-Me-Pseudo UTP, 100mM Solution Application_mRNA " Nucleic acids research 30N1 methyl pseudouridine 5 triphosphate (m1TP) is a chemically modified uridine nucleotide designed for enzymatic incorporation into RNA during IVT using RNA polymerases such as T7 RNA polymerase. m1TP is an N1 methylated derivative of pseudouridine that preserves base pairing properties while altering innate immune recognition. When substituted for uridine in IVT, m1 reduces activation of innate immune sensors and enhances translational efficiency of